ArfGAPs; Depletion or Overexpression?

Mammalian cell overexpression and siRNA depletion

Yip3 was cloned into the mammalian vector pcDNA3.1. HEK293 cells were transfected with pcDNA3.1 human Yip3 or pcDNA3.1, collected 42 h later, lysed by osmotic shock and shearing, and membranes were purified by ultracentrifugation. Membrane recruitment was as published in buffer plus 100 mM (NH4)2SO4, 100 mM GTPgS, 0.1 mg ml 21 BSA, protease inhibitor cocktail (0.34 units ml aprotinin, 0.01 mg m...

Enzymology and Regulation of ArfGAPs and ArfGEFs

ArfGAPs: key regulators for receptor sorting.

Mammalian cells have many membranous organelles that require proper composition of proteins and lipids. Cargo sorting is a process required for transporting specific proteins and lipids to appropriate organelles, and if this process is disrupted, organelle function as well as cell function is disrupted. ArfGAP family proteins have been found to be critical for receptor sorting. In this review, ...

Analysis of gene expression profiles in HeLa cells in response to overexpression or siRNA-mediated depletion of NASP

BACKGROUND NASP (Nuclear Autoantigenic Sperm Protein) is a linker histone chaperone required for normal cell division. Changes in NASP expression significantly affect cell growth and development; loss of gene function results in embryonic lethality. However, the mechanism by which NASP exerts its effects in the cell cycle is not understood. To understand the pathways and networks that may invol...

Memory-Modulation: Self-Improvement or Self-Depletion?

Autobiographical memory is fundamental to the process of self-construction. Therefore, the possibility of modifying autobiographical memories, in particular with memory-modulation and memory-erasing, is a very important topic both from the theoretical and from the practical point of view. The aim of this paper is to illustrate the state of the art of some of the most promising areas of memory-m...